Impact of Low CO <sub>2</sub> Concentration on Autotrophic Production of Poly[( <i>R</i> )-3-hydroxybutyrate] by <i>Ralstonia eutropha</i> H16 and Synergistic Effect of Carbonic Anhydrase
低CO2濃度がRalstonia eutropha H16によるポリ[(R)-3-ヒドロキシブチレート]の独立栄養生産に与える影響と炭酸脱水酵素の相乗効果 (AI 翻訳)
Chih-Ting Wang, Ramamoorthi M Sivashankari, Yuki Miyahara, Takeharu Tsuge
🤖 gxceed AI 要約
日本語
本研究では、水素酸化化学独立栄養細菌Ralstonia eutropha H16を用いて、低CO2濃度条件下でのポリ[(R)-3-ヒドロキシブチレート][P(3HB)]生産を調査。1.4 vol%の低CO2条件でP(3HB)濃度2.71 g/L、含有量77 wt%を達成。さらに炭酸脱水酵素Canの高発現により、低CO2条件下での生産が促進され、P(3HB)濃度2.92 g/L、含有量81 wt%に向上。低CO2条件とCan活性の組合せがP(3HB)生産に相乗効果を示すことを明らかにした。
English
This study investigates the autotrophic production of poly[(R)-3-hydroxybutyrate] (P(3HB)) by Ralstonia eutropha H16 under low CO2 concentrations. At 1.4 vol% CO2, P(3HB) concentration reached 2.71 g/L with 77 wt% content. Overexpression of carbonic anhydrase (Can) further enhanced production to 2.92 g/L and 81 wt% under low CO2, demonstrating a synergistic effect. This work provides insights into CO2 utilization for bioplastic production.
Unofficial AI-generated summary based on the public title and abstract. Not an official translation.
📝 gxceed 編集解説 — Why this matters
日本のGX文脈において
本研究成果は、日本のGX政策におけるCO2の資源化（カーボンリサイクル）技術の一環として注目される。特に、低濃度CO2を含む排出ガスからの直接的なPHA生産は、産業排ガス活用の可能性を示す。ただし、現時点では実験室レベルであり、実用化にはさらなるスケールアップ研究が必要。
In the global GX context
This paper contributes to the global CCUS and bioeconomy literature by demonstrating enhanced bioplastic production under low CO2 conditions, which is relevant for utilizing industrial flue gases. However, the focus is on microbial engineering rather than direct climate policy or disclosure. It offers a technical pathway for carbon capture and utilization, which could be integrated into broader decarbonization strategies.
👥 読者別の含意
🔬研究者:Researchers in microbial engineering and carbon capture utilization should note the synergistic effect of low CO2 and carbonic anhydrase overexpression on P(3HB) production.
🏢実務担当者:Practitioners in bioplastics or CCUS may find the low-CO2 utilization approach relevant for developing scalable processes, though further engineering is needed.
📄 Abstract（原文）
Polyhydroxyalkanoates (PHAs) are bacterial polyesters, and poly[(R)-3-hydroxybutyrate] [P(3HB)] is a member of PHAs, with promising applications as a biodegradable plastic. Ralstonia eutropha H16, a hydrogen-oxidizing chemoautotroph, can biosynthesize P(3HB) using CO2 as a sole carbon source under autotrophic culture conditions. This study investigated the effects of CO2 concentrations on autotrophic cell growth and P(3HB) production when continuously supplying noncombustible gas mixtures composed of H2 (3.8 vol %), O2 (7 vol %), CO2 (1.4–13.4 vol %), and N2 (balance gas). Under low CO2 conditions (1.4 vol %), P(3HB) biosynthesis was significantly enhanced more than under higher CO2 conditions, reaching 2.71 g/L P(3HB) concentration and 77 wt % P(3HB) content after 144 h of cultivation. Furthermore, β-class carbonic anhydrase (Can), an enzyme that catalyzes the reversible hydration of CO2 to bicarbonate in the cell, was expressed at higher levels by using plasmid-based expression. As a result, enhanced P(3HB) biosynthesis was observed only under low CO2 conditions, reaching 2.92 g/L P(3HB) concentration and 81 wt % P(3HB) content. This result suggests that Can plays an important role under low CO2 conditions. The molecular weight of P(3HB) varied with the CO2 concentration of the supplied gas, whereas the increased Can expression had almost no effect. This study demonstrates that low CO2 conditions combined with increased Can expression synergistically promote P(3HB) biosynthesis in R. eutropha H16.
🔗 Provenance — このレコードを発見したソース
- openalex https://doi.org/10.1021/acssuschemeng.6c00126first seen 2026-05-17 06:26:59
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