CO2 availability as process tool to enhance isobutyric acid production in methanol fermentation by Clostridium luticellarii.
Clostridium luticellariiによるメタノール発酵におけるイソ酪酸生産促進のためのプロセスツールとしてのCO2利用可能性 (AI 翻訳)
Camille Petrognani, Q. Mariën, Lander De Vos, Merlijn van Oijen, Nico Boon, Ramón Ganigué
🤖 gxceed AI 要約
日本語
本論文は、CO2由来メタノール発酵において、CO2供給量の制御がClostridium luticellariiの代謝をイソ酪酸生産に振り向けることを明らかにした。CO2制限条件下では酢酸からイソ酪酸へのフラックスが増大し、最高で全生成物の41%に達した。実験を3Lバイオリアクターで再現し、流加培養により2.70 g/Lのイソ酪酸を達成。CCUプロセスの効率化に寄与する。
English
This paper shows that controlling CO2 availability in methanol fermentation redirects metabolism of Clostridium luticellarii towards isobutyric acid production, achieving up to 41% of total products under CO2 limitation. Fed-batch cultivation yielded 2.70 g/L isobutyric acid, demonstrating a viable operational tool for carbon capture and utilization (CCU).
Unofficial AI-generated summary based on the public title and abstract. Not an official translation.
📝 gxceed 編集解説 — Why this matters
日本のGX文脈において
日本のグリーン成長戦略ではCO2資源化が重点分野であり、本成果はバイオベースの炭素循環技術として産業応用が期待される。特に化学品原料の脱炭素化に寄与する可能性がある。
In the global GX context
This study contributes to the global CCU research by demonstrating a biotechnological route to convert CO2 and methanol into a valuable chemical, isobutyric acid. It offers a process lever for integrating CO2 utilization into chemical production, relevant to circular carbon economy goals.
👥 読者別の含意
🔬研究者:Provides mechanistic insights into CO2-driven metabolic shifts in acetogens, useful for designing CCU bioprocesses.
🏢実務担当者:Offers a potential operational strategy for chemical companies to produce green chemicals from CO2 and methanol.
🏛政策担当者:Highlights the feasibility of biological CCU, supporting policies that promote carbon recycling technologies.
📄 Abstract（原文）
The bioconversion of CO2‑derived methanol into higher‑value chemicals offers an attractive route for hybrid catalytic-biotechnological carbon capture and utilization (CCU). Clostridium luticellarii is one of the few acetogens able to produce isobutyric acid. However, operational and metabolic factors driving its production are poorly understood. This work investigates how CO2 availability shapes the product spectrum of C. luticellarii during methylotrophic growth and assesses whether CO2 supply can be used as a process lever to promote isobutyric acid formation. Batch experiments with varying initial bicarbonate concentrations revealed that conditions leading to CO2 limitation (i.e., DIC depletion at ≤ 30 mM NaHCO3) redirected carbon and electron fluxes away from acetic acid toward butyric and isobutyric acids, with the latter accounting for up to 41% of total products. This metabolic switch was not observed when CO2 was in excess (>45 mM). High acetic acid supplementation (100 mM) triggered isobutyric acid production even while CO2 was still available, indicating a combined regulation of dissolved inorganic carbon (DIC) and acetic acid availability. Net acetic acid consumption took place in all isobutyric acid-producing experiments. These observations were reproduced in 3-L bioreactors and further exploited through a fed‑batch strategy in which an initial acetic‑acid‑accumulating phase was followed by CO2‑limited feeding. This approach achieved complete conversion of methanol and CO2 and yielded an isobutyric acid titer of 2.70 ± 0.04 g·L-1. Controlling CO2 availability is a viable operational tool to steer C. luticellarii metabolism toward isobutyric acid production, in interaction with electron acceptor availability.
🔗 Provenance — このレコードを発見したソース
- semanticscholar https://doi.org/10.1016/j.biortech.2026.135255first seen 2026-06-30 05:44:17
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