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Carbonic anhydrase activity under low zinc in <i>Phaeodactylum tricornutum</i> largely depends on metal-free iota-carbonic anhydrase

低亜鉛条件下での<i>Phaeodactylum tricornutum</i>における炭酸脱水酵素活性は主に金属非依存性イオタ炭酸脱水酵素に依存する (AI 翻訳)

Luisana Avilán, Cassy Gérard, Anaïs Scholivet, Gilles Peltier, Régine Lebrun, Vincent Fourmond, Chloé Guendon, Marta Carletti, Yannick Sérès, Juliette Salvaing, Véronique Receveur‐Brechot, Alberto Amato, Stephen C. Maberly, Brigitte Gontero, Hélène Launay

PLANT PHYSIOLOGY📚 査読済 / ジャーナル2026-07-18#気候科学Origin: EU
DOI: 10.1093/plphys/kiag510
原典: https://doi.org/10.1093/plphys/kiag510

🤖 gxceed AI 要約

日本語

本研究は、珪藻<i>Phaeodactylum tricornutum</i>が新規のイオタクラス炭酸脱水酵素（Pt-ιCA）を持ち、金属補因子なしで触媒活性を示すことを発見した。低亜鉛条件下で発現が誘導され、CO2濃縮機構において重要な役割を果たす。この酵素は金属制限環境における進化的優位性を示唆する。

English

This study identifies a novel iota-class carbonic anhydrase (Pt-ιCA) in the diatom Phaeodactylum tricornutum that exhibits catalytic activity without a metal cofactor. It is induced under low zinc conditions and plays a role in CO2 concentrating mechanisms, suggesting an evolutionary advantage in metal-limited environments.

Unofficial AI-generated summary based on the public title and abstract. Not an official translation.

📝 gxceed 編集解説 — Why this matters

日本のGX文脈において

日本は海洋バイオテクノロジーと二酸化炭素回収に関心があり、本研究成果は生物学的炭素固定メカニズムの理解を深め、将来的な応用の基礎となりうる。

In the global GX context

This fundamental discovery of a metal-free carbonic anhydrase could inform bio-inspired carbon capture technologies and enhance understanding of marine carbon cycling, relevant to global climate mitigation strategies.

👥 読者別の含意

🔬研究者:Provides insights into a novel metal-free carbonic anhydrase mechanism that could inspire biotechnological applications in carbon capture or synthetic biology.

📄 Abstract（原文）

Carbonic anhydrases (CA) typically possess a Zn2+-cofactor, and are involved in a wide range of adaptative metabolisms involving pH and dissolved inorganic carbon homeostasis. In photosynthetic microorganisms, they are involved in CO2 concentrating mechanisms (CCM) that supply CO2 to Rubisco. Here we show that the model diatom Phaeodactylum tricornutum possesses a CA from the newly identified iota class (Pt-ιCA) that exhibited catalytic activity without a metal cofactor, in contrast to most CAs. Metal-free Pt-ιCA activity was two orders of magnitude lower than that the Zn2+ containing bovine erythrocyte CA, yet still in the range of expected value for CA. Zn2+ concentrations on the picomolar range induced the expression of Pt-ιCA in P. tricornutum, together with the Zn2+/Co2+ responsive protein A. Zn2+ scarcity did not prevent the induction of Zn2+-containing CAs involved in biophysical CCM when the cells were transferred from high pCO2 to very low pCO2 conditions. The induction of CCM at very low pCO2 and low Zn2+ conditions was concomitant to induction of light harvesting complex and remodeling of the pentose phosphate pathways. The total cellular CA activity decreased in Pt-ιCA deletion mutant (ΔιCA) compared to control cells, indicating that Pt-ιCA is active in P. tricornutum under low Zn2+ conditions. P. tricornutum adapted to the absence of Pt-ιCA by a morphometric change, without affecting photosynthesis or growth rate. The newly identified metal-free Pt-ιCA represents an evolutionary advantage for diatoms that live in metal-limited environments.

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